PROGRAM
TUESDAY July 14th
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Introduction to the course
Afternoon (15.00-18.00)
Welcome to students and little Introduction to the course (prof. R. Danovaro, President of SZN).
Theoretic Lecture:
Constrains that structure exerts on function and evolution of proteins, evolutionary divergence and convergence of proteins, evolution of protein complexity (protein families); enzyme promiscuity, moonlighting function of proteins, examples from the sea (Dr. I. Castellano; prof. A. Peracchi).
Social Dinner
(to be defined)
WEDNESDAY, July 15th
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Evolution of metabolic pathways
(Prof. A. Peracchi)
Morning (10.00-12.00)
Theoretic Lecture:
The limits of enzyme specificity: liabilities and opportunities in the evolution of metabolism.
Afternoon (15.00-18.00)
Practical session:
Practical Lesson: Databases of metabolic pathways (MetaCyc, KEGG) and of enzyme activities (BRENDA). Exercises on filling gaps in predicted metabolic pathways and on identifying candidates for orphan activities.
THURSDAY July 16th
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Metabolic engineering of bacteria (Dr. Jae-Seong Yang)
Morning (10.00-12.00)
Theoretic Lecture:
Regulative mechanisms of gene expression control and protein translation in bacteria.
Coffee break
Examples of metabolic pathways optimization using high-throughput methods.
Afternoon (15.00-18.00)
Practical session:
Introduction and application of ELM-seq technology (expression level monitoring by DNA methylation): a novel reporter system coupled with high-throughput sequencing. UTRDesigner and UTRLibrary to perform predictive design of mRNA translation initiation region for metabolic engineering in bacteria.